Dunce mutants of Drosophila melanogaster: mutants defective in the cyclic AMP phosphodiesterase enzyme system

The cyclic AMP and cyclic GMP phosphodiesterase activities present in flies of six mutant strains of the dunce gene and in the parent wild- type strains are characterized. All of the mutants exhibit aberrant cyclic AMP metabolism. The mutant strains dunceM14, dunceM11, and dunceML appear to be amorphic, because they completely lack the cAMP-specific phosphodiesterase normally present in adult flies. These strains exhibit extremely high levels of cAMP. The mutant strains dunce1, dunce2, and dunceCK are hypomorphic and exhibit reduced levels of the cAMP-specific phosphodiesterase. These strains exhibit less marked increases in cAMP content compared with the three amorphic strains. The dunce2 strain possesses a residual enzyme activity that exhibits anomalous kinetics compared with those of the normal enzyme. The possibility that the dunce locus is the structural gene for the cAMP-specific phosphodiesterase is discussed

Spray Cooling Trajectory Angle Impact Upon Heat Flux Using a Straight Finned Enhanced Surface

Experiments were conducted to study the effects of spray trajectory angles upon heat flux for flat and enhanced surface spray cooling. The surface enhancement consisted of straight fins machined on the top surface of a copper heater block. Spray cooling curves were obtained with the straight fin surface aligned both parallel (axial) and perpendicular (transverse) to the spray axis. Measurements were also obtained on a flat surface heater block for comparison purposes. Each copper block had a cross-sectional area of 2.0 sq cm. A 2x2 nozzle array was used with PF-5060 as the working fluid. Thermal performance data was obtained under nominally degassed (chamber pressure of 41.4 kPa) conditions. Results show that the maximum CHF in all cases was attained for a trajectory angle of 30' from the surface normal. Furthermore, trajectory angles applied to straight finned surfaces can have a critical heat flux (CHF) enhancement as much as 75% (heat flux value of 140 W/sq cm) relative to the vertical spray orientation for the analogous flat surface case under nominally degassed conditions

Impact of Cubic Pin Finned Surface Structure Geometry upon Spray Cooling Heat Transfer

Experiments were conducted to study the effects of enhanced surface structures on heat flux using spray cooling. The surface enhancements consisted of cubic pin fins machined on the top surface of copper heater blocks. The structure height, pitch, and width were parametrically vaned. Each copper block had a projected cross-sectional area of 2.0 sq cm. Measurements were also obtained on a heater block with a flat surface for baseline comparison purposes. A 2 x 2 nozzle array was used with PF-5060 as the working fluid. Thermal performance data were obtained under nominally degassed (chamber pressure of 41.4 kPa) and gassy conditions (chamber with N2 gas at 100.7 kPa) with a bulk fluid temperature of 20.5 C. Results for both the degassed and gassy cases show that structure width and separation distance have a dominant effect upon the heat transfer for the size ranges used. Cubic pin fin height had little impact upon heat flux. The maximum critical heat flux (CHF) attained for any of the surfaces was 121 W/sq cm, giving an enhancement of 51% relative to the flat surface case under nominally degassed conditions. The gassy case had a maximum CHF of 149 W/sq cm, giving an enhancement of 38% relative to the flat surface case

Enhanced Surface Spray Cooling with Embedded and Compound Extended Surface Structures

Experiments were conducted to study the effects of enhanced surface structures on heat flux using spray cooling. The surface enhancements consisted of embedded structures (dimples, pores, and tunnels) and compound extended surface enhancements (straight fins, cubic pin fins and dimples) machined on and within the top surface of copper heater blocks. Each copper block had a projected cross-sectional area of 2.0 sq cm. Measurements were also obtained on a heater block with a flat surface for baseline comparison purposes. A 2x2 nozzle array was used with PF-5060 as the working fluid. Thermal performance data was obtained under nominally degassed (chamber pressure of 41.4 kPa) and gassy conditions (chamber with N2 gas at 101 kPa) with a bulk fluid temperature of 20.5 C. Results for both the nominally degassed and gassy cases show that the highest critical heat flux (CHF) was attained using straight fins and porous tunnels. For the nominally degassed case, both had a CHF of approx.: 142 W/sq cm while for the gassy case their CHF values increased to 175 W/sq cm. This gave an enhancement relative to the nominally degassed flat surface case of approx. 77% and 119% respectively

Changing Bacterial Growth Efficiencies across a Natural Nutrient Gradient in an Oligotrophic Estuary

Recent studies have characterized coastal estuarine systems as important components of the global carbon cycle. This study investigated carbon cycling through the microbial loop of Florida Bay by use of bacterial growth efficiency calculations. Bacterial production, bacterial respiration, and other environmental parameters were measured at three sites located along a historic phosphorus-limitation gradient in Florida Bay and compared to a relatively nutrient enriched site in Biscayne Bay. A new method for measuring bacterial respiration in oligotrophic waters involving tracing respiration of 13C-glucose was developed. The results of the study indicate that 13C tracer assays may provide a better means of measuring bacterial respiration in low nutrient environments than traditional dissolved oxygen consumption-based methods due to strong correlations between incubation length and δ13C values. Results also suggest that overall bacterial growth efficiency may be lower at the most nutrient limited sites

K band Spectroscopy of Ultraluminous Infrared Galaxies: The 2 Jy Sample

We present near-infrared spectroscopy for a complete sample of 33 ultraluminous infrared galaxies at a resolution of R\approx 1000. Most of the wavelength range from 1.80-2.20 microns in the rest frame is covered, including the Pa-alpha and Br-gamma hydrogen recombination lines, and the molecular hydrogen vibration-rotation 1-0 S(1) and S(3) lines. Other species, such as He I, [Fe II], and [Si VI] appear in the spectra as well, in addition to a number of weaker molecular hydrogen lines. Nuclear extractions for each of the individual galaxies are presented here, along with spectra of secondary nuclei, where available. The Pa-alpha emission is seen to be highly concentrated on the nuclei, typically with very little emision extending beyond a radius of 1 kpc. Signatures of active nuclei are rare in the present sample, occurring in only two of the 33 galaxies. It is found that visual extinctions to the nuclei via the Pa-alpha/Br-gamma line ratio in excess of 10 magnitudes are relatively common among ULIRGs, and that visual extinctions greater than 25 mag are necessary to conceal a QSO emitting half the total bolometric luminosity. The vibration-rotation lines of molecular hydrogen appear to be predominantly thermal in origin, with effective temperatures generally around 2200 K. The relative nuclear velocities between double nucleus ULIRGs are investigated, through which it is inferred that the maximum deprojected velocity difference is about 200 km/s. This figure is lower than the velocities predicted by physical models of strong interactions/mergers of large, gas-rich galaxies.Comment: 52 pages (19 with just figures), 9 figures, accepted for publication in the Astronomical Journal; Table 3 not formatted properly on astro-ph: get source and print Murphy.tab3.p

Genetic screen in Drosophila muscle identifies autophagy-mediated T-tubule remodeling and a Rab2 role in autophagy.

Transverse (T)-tubules make-up a specialized network of tubulated muscle cell membranes involved in excitation-contraction coupling for power of contraction. Little is known about how T-tubules maintain highly organized structures and contacts throughout the contractile system despite the ongoing muscle remodeling that occurs with muscle atrophy, damage and aging. We uncovered an essential role for autophagy in T-tubule remodeling with genetic screens of a developmentally regulated remodeling program in Drosophila abdominal muscles. Here, we show that autophagy is both upregulated with and required for progression through T-tubule disassembly stages. Along with known mediators of autophagosome-lysosome fusion, our screens uncovered an unexpected shared role for Rab2 with a broadly conserved function in autophagic clearance. Rab2 localizes to autophagosomes and binds to HOPS complex members, suggesting a direct role in autophagosome tethering/fusion. Together, the high membrane flux with muscle remodeling permits unprecedented analysis both of T-tubule dynamics and fundamental trafficking mechanisms

A functional genomic analysis of cell morphology using RNA interference

BACKGROUND: The diversity of metazoan cell shapes is influenced by the dynamic cytoskeletal network. With the advent of RNA-interference (RNAi) technology, it is now possible to screen systematically for genes controlling specific cell-biological processes, including those required to generate distinct morphologies. RESULTS: We adapted existing RNAi technology in Drosophila cell culture for use in high-throughput screens to enable a comprehensive genetic dissection of cell morphogenesis. To identify genes responsible for the characteristic shape of two morphologically distinct cell lines, we performed RNAi screens in each line with a set of double-stranded RNAs (dsRNAs) targeting 994 predicted cell shape regulators. Using automated fluorescence microscopy to visualize actin filaments, microtubules and DNA, we detected morphological phenotypes for 160 genes, one-third of which have not been previously characterized in vivo. Genes with similar phenotypes corresponded to known components of pathways controlling cytoskeletal organization and cell shape, leading us to propose similar functions for previously uncharacterized genes. Furthermore, we were able to uncover genes acting within a specific pathway using a co-RNAi screen to identify dsRNA suppressors of a cell shape change induced by Pten dsRNA. CONCLUSIONS: Using RNAi, we identified genes that influence cytoskeletal organization and morphology in two distinct cell types. Some genes exhibited similar RNAi phenotypes in both cell types, while others appeared to have cell-type-specific functions, in part reflecting the different mechanisms used to generate a round or a flat cell morphology

Fabrication and transport critical currents of multifilamentary MgB2/Fe wires and tapes

Multifilamentary MgB2/Fe wires and tapes with high transport critical current densities have been fabricated using a straightforward powder-in-tube (PIT) process. After annealing, we measured transport jc values up to 1.1 * 105 A/cm2 at 4.2 K and in a field of 2 T in a MgB2/Fe square wire with 7 filaments fabricated by two-axial rolling, and up to 5 * 104 A/cm2 at 4.2 K in 1 T in a MgB2/Fe tape with 7 filaments. For higher currents these multifilamentary wires and tapes quenched due to insufficient thermal stability of filaments. Both the processing routes and deformation methods were found to be important factors for fabricating multifilamentary MgB2 wires and tapes with high transport jc values.Comment: 13 pages, 7 figure